AccuFFPE DNA Preparation Kit

AccuPrep Kit

AccuFFPE DNA Preparation Kit

The AccuFFPE DNA Preparation Kit is a spin column-type kit that is the most universally known method for DNA extraction and purification. The HiDeparaffinization Solution included in the kit minimizes DNA damage by removing the paraffin which leads to a relatively large amount of extracted DNA. This feature makes this kit ideal for amplification in the target region. This kit can also be used to remove impurities (e.g. inhibitors, contaminants, cell debris, etc.) and extract highly concentrated and high-quality DNA.
Cat : ACN 03.50
For the rapid preparation of high quality DNA from small amounts of FFPE tissues
  • Input Amount

    5 ~ 10 µm thickness
  • Processing Time

    < 2.5 hours (incl. lysis time)
  • Format

    Column
  • Elution Volume

    20 ~ 100 ㎕
  • Yield

    ≥ 1 ng
  • Purity(A260/A280)

    1.7 ~ 1.9

Product Highlights

  • Convenience

    User-friendly features
  • Small Input Amount

    DNA extraction possible from small amounts of FFPE samples
  • High Concentration & High Purity

    Highly concentrated and high-quality cfDNA extraction
  • Easy

    Spin column-type format makes the kit user friendly
  • Safe

    No organic solvents make it safe to use (HiDeparaffinization Solution)
  • Patent Application

    First commercial product and patent in South Korea
  • Application

    PCR, Real Time PCR, Sequencing, Microarray, etc.

Experimental Procedure

  • STEP 01

    Section or Homogenize

  • STEP 02

    Take sample(5 ~ 10 ㎛ paraffin section)

  • STEP 03

    Add Hideparaffinization Solution

  • STEP 04

    Add Proteinase K & Lysis buffer

  • STEP 05

    Incubation at 56 ℃ for 1 - 2 hr

  • STEP 06

    Incubation at 90 ℃ for 1 - 2 hr

  • STEP 07

    Take the blue lower phase solution

  • STEP 01

    Add Binding buffer

  • STEP 02

    DNA Binding

  • STEP 03

    Add Washing buffer ①

  • STEP 04

    Add Washing buffer ①

  • STEP 05

    Drying

  • STEP 06

    Add Elution buffer

  • STEP 07

    Purified DNA

Experimental Data

  • Address.

    GDX Lab Inc. 770 West Hampden Avenue Suite 125 Englewood, Colorado, 80110 United States of America
  • Tel.

    720-255-2280
  • E-mail.

    info@gdxlab.com