16S/18S/ITS Microbiome Analysis

Unlike the genomic analysis that examines the human genome which stays fixed throughout one’s life, the microbiome analysis
reflects the microbiome status of the user at the time of sample collection taking into account variable factors such as age, diet,
hereditary diseases, and environment which is also useful for understanding the user’s microorganism levels.

16S/18S/ITS Microbiome Analysis

What is the 16S/18S/ITS
Microbiome Analysis Service?
The 16S/18S/ITS Microbiome Analysis identifies various microbiota/eukaryote/fungi (e.g. composition and distribution) in the collected samples to generate a customized microbiome profile.

Areas of Analysis

Biota	Analysis Area
Archaea, Bacteria	16S V1V2
	16S V3V4
	16S V4
Eukaryote	18S V3V4
Eukaryote	18S V9
Fungi	ITS1
Fungi	ITS2

Standard Analysis ltems

Standard Analysis Items (For analysis on the ITS2 region, items marked with * are excluded from the results.)

1. NGS QC Data
2. Taxonomic Distribution
– Bar plots for relative ratio of microbes in phylum, class, order, family, genus, and species level
3. Alpha Diversity
– Chao1, Observed Species, Shannon Index,
Simpson Index, PD Whole Tree*

4. Rarefaction Curves
– Chao1, Observed Species, Shannon Index, Simpson Index, PD Whole Tree*
5. Beta Diversity
– Bray-Curtis*, Unweighted Unifrac*, Weighted Unifrac*
6. UPGMA Trees
– Bray-Curtis*, Unweighted Unifrac*, Weighted Unifrac*

Additional Analysis

1. LEfSe 2. XOR 3. PICRUSt

Experimental Data